TB Lamp Technique

World Health Organization End TB Strategy calls for the early diagnosis of tuberculosis (TB) and for universal drug-susceptibility testing, highlighting the critical role of laboratories in the post-2015 era in rapidly and accurately detecting TB and drug resistance.

Molecular assays based on nucleic acid amplification techniques, such as polymerase chain reaction, have been developed for rapid TB diagnosis and are being implemented in developing countries.

Loop-mediated isothermal amplification (LAMP) is a unique, temperature-independent technique for amplifying DNA that is simple to use, providing a visual display that is easy to read

The test is robust and does not require sophisticated instrumentation and can be used at a peripheral health centre level, under biosafety requirements similar to microscopy.

LAMP methods have been used to detect malaria and several neglected tropical diseases. Similar training as for microscopy is required.

 

Specially designed as a consolidated platform for sample preparation, amplification and easy visual result reading, HumaLoop T facilitates sensitive and reliable detection of tuberculosis with the LoopampTM MTBC Detection Kit.

- For small to medium throughput up to 16 tests per run or up to 70 samples per day

- Preinstalled and fixed incubation times and temperatures for LoopampTM assays

- Consolidated processing: sample preparation, amplification and detection on a single instrument

- Perfect for use in remote areas with independent power solution by solar panel and battery system

- Explicit interpretation by visual reading of fluorescence signals

- Fast reporting: results in 1 – 2 hours

 

The HumaTurb system allows real-time detection of turbidity based upon magnesium pyrophosphate which is generated during the amplification process. The entire system consists of HumaTurb C + A. The HumaTurb C for the setup and control of incubation time and temperature, necessary for amplification. The amplification itself takes place in the second part of the system, the HumaTurb A. The sample lysis is performed with the HumaHeat.

- For medium to high throughput: up to 96 tests per run if expanded with 6 HumaTurb A units

- Different LoopampTM assays can be performed in one run

- Flexible data transfer via USB and LIS connectivity

- Built-in printer

- Automated result reporting

 

HumaLoop T

 

HumaTurb C with 6 HumaTurb A Units

 

 

 

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